Hydroxyapatite and Demineralized Bone Matrix from Marine Food Waste – A Possible Bone Implant
Rethinam Senthil,
Sathyaraj Weslen Vedakumari,
Thotapalli Parvathaleswara Sastry
Issue:
Volume 3, Issue 1, March 2018
Pages:
1-6
Received:
28 September 2017
Accepted:
18 October 2017
Published:
18 January 2018
Abstract: In the present study, a novel bone implant (BI) was prepared using demineralized bone matrix (DBM) and hydroxyapatite (HA) isolated from Bluefin trevally (BT) bones, which was considered to be a marine industry food waste. Gelatin (GA) was used as a binder. Physico-chemical characterization and in vitro studies were carried out using this implant. Fourier transform infrared spectrum of BI exhibited the characteristic bands of all the three components viz., DBM, HA and GA, while scanning electron microscopic studies revealed the irregular shape of the particles. The mechanical properties of BI were also appreciable. In vitro studies were carried out using Human keratinocyte cell line (HaCaT), wherein MTT (3-(4,5-dimethylazol-2-yl)-2,5-diphenyl-tetrazolium bromide) assay proved the biocompatibility of BI. From the results obtained it could be stated that BI prepared from waste marine bones could serve as a promising biomaterial for bone tissue engineering applications.
Abstract: In the present study, a novel bone implant (BI) was prepared using demineralized bone matrix (DBM) and hydroxyapatite (HA) isolated from Bluefin trevally (BT) bones, which was considered to be a marine industry food waste. Gelatin (GA) was used as a binder. Physico-chemical characterization and in vitro studies were carried out using this implant. ...
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