Epidemiology of Adenovirus and Genotypic Characteristics of Group A Rotavirus Among Diarrhoeic Children Aged 0 to 5 Years Old in the Ngaoundere Region of Cameroon

Kagning Tsinda Emmanuel; Fokunang Charles Ntungwen; Tchuenguem Fohouo Fernand-Nestor; Nwabo Kamdje Armel Herve; Nukenine Elias Nchinwan

doi:doi:10.11648/j.sjph.20150304.21

Epidemiology of Adenovirus and Genotypic Characteristics of Group A Rotavirus Among Diarrhoeic Children Aged 0 to 5 Years Old in the Ngaoundere Region of Cameroon

Kagning Tsinda Emmanuel, Fokunang Charles Ntungwen, Tchuenguem Fohouo Fernand-Nestor, Nwabo Kamdje Armel Herve, Nukenine Elias Nchinwan

Published in Science Journal of Public Health (Volume 3, Issue 4)

Received: 13 April 2015 Accepted: 29 April 2015 Published: 6 June 2015

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Abstract

In the context of recent introduction of a Rotavirus vaccine, little is known about the epidemiology of Rotavirus and Adenovirus gastroenteritis in Cameroon in general and in Ngaoundere specifically. The main objective of this study was to determine the frequency of Rotavirus and Adenovirus antigens as well as to characterise circulating Rotavirus strains in children with diarrhoea in Ngaoundere. A convenience sampling method was used and stool samples collected from patients consulting at the study sites from April to July 2014 were subjected to a qualitative immunochromatographic test to detect group antigens of group A Rotavirus and Adenovirus. Socio-demographic data were collected using a pre-tested questionnaire. Stool samples tested positive for Group A Rotavirus were genotyped by multiplex nested RT-PCR method. Logistic regression, Chi-square or Fisher’s exact tests were performed when appropriate. P-value < 0.05 at 95% confidence interval was considered statistically significant. Adenovirus antigen was detected in 26.4% (56) of children, whereas, group A Rotavirus was detected in 2.3% (5); the co-infection rate was 5.3%. Vomiting, fever, moderate dehydration, severe dehydration and the presence of polymorphonuclear cells in stool were significantly associated with the presence of Adenovirus antigen in stool. Group A Rotavirus was also found to circulate in Ngaoundere and the G1P[8] genotype showed indication of being the most represented. Adenovirus antigen was shown to be an important agent in childhood acute gastroenteritis in Ngaoundere and this study has been used as a baseline data for this infection in this region of Cameroon. The appropriate management of viral acute gastroenteritis necessitates the detection of Rotavirus and Adenovirus in clinical routine practice. Furthermore, Rotarix™ vaccination should be encouraged and the populations should be educated on hygiene practices.

Published in	Science Journal of Public Health (Volume 3, Issue 4)
DOI	10.11648/j.sjph.20150304.21
Page(s)	525-537
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Rotavirus, Adenovirus, Epidemiology, Genotypes, Children Aged 0 to 5 Years

References

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APA Style

Kagning Tsinda Emmanuel, Fokunang Charles Ntungwen, Tchuenguem Fohouo Fernand-Nestor, Nwabo Kamdje Armel Herve, Nukenine Elias Nchinwan. (2015). Epidemiology of Adenovirus and Genotypic Characteristics of Group A Rotavirus Among Diarrhoeic Children Aged 0 to 5 Years Old in the Ngaoundere Region of Cameroon. Science Journal of Public Health, 3(4), 525-537. https://doi.org/10.11648/j.sjph.20150304.21

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ACS Style

Kagning Tsinda Emmanuel; Fokunang Charles Ntungwen; Tchuenguem Fohouo Fernand-Nestor; Nwabo Kamdje Armel Herve; Nukenine Elias Nchinwan. Epidemiology of Adenovirus and Genotypic Characteristics of Group A Rotavirus Among Diarrhoeic Children Aged 0 to 5 Years Old in the Ngaoundere Region of Cameroon. Sci. J. Public Health 2015, 3(4), 525-537. doi: 10.11648/j.sjph.20150304.21

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AMA Style

Kagning Tsinda Emmanuel, Fokunang Charles Ntungwen, Tchuenguem Fohouo Fernand-Nestor, Nwabo Kamdje Armel Herve, Nukenine Elias Nchinwan. Epidemiology of Adenovirus and Genotypic Characteristics of Group A Rotavirus Among Diarrhoeic Children Aged 0 to 5 Years Old in the Ngaoundere Region of Cameroon. Sci J Public Health. 2015;3(4):525-537. doi: 10.11648/j.sjph.20150304.21

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@article{10.11648/j.sjph.20150304.21,
  author = {Kagning Tsinda Emmanuel and Fokunang Charles Ntungwen and Tchuenguem Fohouo Fernand-Nestor and Nwabo Kamdje Armel Herve and Nukenine Elias Nchinwan},
  title = {Epidemiology of Adenovirus and Genotypic Characteristics of Group A Rotavirus Among Diarrhoeic Children Aged 0 to 5 Years Old in the Ngaoundere Region of Cameroon},
  journal = {Science Journal of Public Health},
  volume = {3},
  number = {4},
  pages = {525-537},
  doi = {10.11648/j.sjph.20150304.21},
  url = {https://doi.org/10.11648/j.sjph.20150304.21},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjph.20150304.21},
  abstract = {In the context of recent introduction of a Rotavirus vaccine, little is known about the epidemiology of Rotavirus and Adenovirus gastroenteritis in Cameroon in general and in Ngaoundere specifically. The main objective of this study was to determine the frequency of Rotavirus and Adenovirus antigens as well as to characterise circulating Rotavirus strains in children with diarrhoea in Ngaoundere. A convenience sampling method was used and stool samples collected from patients consulting at the study sites from April to July 2014 were subjected to a qualitative immunochromatographic test to detect group antigens of group A Rotavirus and Adenovirus. Socio-demographic data were collected using a pre-tested questionnaire. Stool samples tested positive for Group A Rotavirus were genotyped by multiplex nested RT-PCR method. Logistic regression, Chi-square or Fisher’s exact tests were performed when appropriate. P-value < 0.05 at 95% confidence interval was considered statistically significant. Adenovirus antigen was detected in 26.4% (56) of children, whereas, group A Rotavirus was detected in 2.3% (5); the co-infection rate was 5.3%. Vomiting, fever, moderate dehydration, severe dehydration and the presence of polymorphonuclear cells in stool were significantly associated with the presence of Adenovirus antigen in stool. Group A Rotavirus was also found to circulate in Ngaoundere and the G1P[8] genotype showed indication of being the most represented. Adenovirus antigen was shown to be an important agent in childhood acute gastroenteritis in Ngaoundere and this study has been used as a baseline data for this infection in this region of Cameroon. The appropriate management of viral acute gastroenteritis necessitates the detection of Rotavirus and Adenovirus in clinical routine practice. Furthermore, Rotarix™ vaccination should be encouraged and the populations should be educated on hygiene practices.},
 year = {2015}
}

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TY  - JOUR
T1  - Epidemiology of Adenovirus and Genotypic Characteristics of Group A Rotavirus Among Diarrhoeic Children Aged 0 to 5 Years Old in the Ngaoundere Region of Cameroon
AU  - Kagning Tsinda Emmanuel
AU  - Fokunang Charles Ntungwen
AU  - Tchuenguem Fohouo Fernand-Nestor
AU  - Nwabo Kamdje Armel Herve
AU  - Nukenine Elias Nchinwan
Y1  - 2015/06/06
PY  - 2015
N1  - https://doi.org/10.11648/j.sjph.20150304.21
DO  - 10.11648/j.sjph.20150304.21
T2  - Science Journal of Public Health
JF  - Science Journal of Public Health
JO  - Science Journal of Public Health
SP  - 525
EP  - 537
PB  - Science Publishing Group
SN  - 2328-7950
UR  - https://doi.org/10.11648/j.sjph.20150304.21
AB  - In the context of recent introduction of a Rotavirus vaccine, little is known about the epidemiology of Rotavirus and Adenovirus gastroenteritis in Cameroon in general and in Ngaoundere specifically. The main objective of this study was to determine the frequency of Rotavirus and Adenovirus antigens as well as to characterise circulating Rotavirus strains in children with diarrhoea in Ngaoundere. A convenience sampling method was used and stool samples collected from patients consulting at the study sites from April to July 2014 were subjected to a qualitative immunochromatographic test to detect group antigens of group A Rotavirus and Adenovirus. Socio-demographic data were collected using a pre-tested questionnaire. Stool samples tested positive for Group A Rotavirus were genotyped by multiplex nested RT-PCR method. Logistic regression, Chi-square or Fisher’s exact tests were performed when appropriate. P-value < 0.05 at 95% confidence interval was considered statistically significant. Adenovirus antigen was detected in 26.4% (56) of children, whereas, group A Rotavirus was detected in 2.3% (5); the co-infection rate was 5.3%. Vomiting, fever, moderate dehydration, severe dehydration and the presence of polymorphonuclear cells in stool were significantly associated with the presence of Adenovirus antigen in stool. Group A Rotavirus was also found to circulate in Ngaoundere and the G1P[8] genotype showed indication of being the most represented. Adenovirus antigen was shown to be an important agent in childhood acute gastroenteritis in Ngaoundere and this study has been used as a baseline data for this infection in this region of Cameroon. The appropriate management of viral acute gastroenteritis necessitates the detection of Rotavirus and Adenovirus in clinical routine practice. Furthermore, Rotarix™ vaccination should be encouraged and the populations should be educated on hygiene practices.
VL  - 3
IS  - 4
ER  -

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Author Information

Kagning Tsinda Emmanuel

Department of Biomedical Sciences, University of Ngaoundere, Ngaoundere, Cameroon
Fokunang Charles Ntungwen

Department of Pharmacotoxicology and pharmacokinetics, University of Yaounde I, Yaounde, Cameroon
Tchuenguem Fohouo Fernand-Nestor

Department of Biological Sciences, University of Ngaoundere, Ngaoundere, Cameroon
Nwabo Kamdje Armel Herve

Department of Biomedical Sciences, University of Ngaoundere, Ngaoundere, Cameroon
Nukenine Elias Nchinwan

Department of Biological Sciences, University of Ngaoundere, Ngaoundere, Cameroon

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APA Style

Kagning Tsinda Emmanuel, Fokunang Charles Ntungwen, Tchuenguem Fohouo Fernand-Nestor, Nwabo Kamdje Armel Herve, Nukenine Elias Nchinwan. (2015). Epidemiology of Adenovirus and Genotypic Characteristics of Group A Rotavirus Among Diarrhoeic Children Aged 0 to 5 Years Old in the Ngaoundere Region of Cameroon. Science Journal of Public Health, 3(4), 525-537. https://doi.org/10.11648/j.sjph.20150304.21

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ACS Style

Kagning Tsinda Emmanuel; Fokunang Charles Ntungwen; Tchuenguem Fohouo Fernand-Nestor; Nwabo Kamdje Armel Herve; Nukenine Elias Nchinwan. Epidemiology of Adenovirus and Genotypic Characteristics of Group A Rotavirus Among Diarrhoeic Children Aged 0 to 5 Years Old in the Ngaoundere Region of Cameroon. Sci. J. Public Health 2015, 3(4), 525-537. doi: 10.11648/j.sjph.20150304.21

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AMA Style

Kagning Tsinda Emmanuel, Fokunang Charles Ntungwen, Tchuenguem Fohouo Fernand-Nestor, Nwabo Kamdje Armel Herve, Nukenine Elias Nchinwan. Epidemiology of Adenovirus and Genotypic Characteristics of Group A Rotavirus Among Diarrhoeic Children Aged 0 to 5 Years Old in the Ngaoundere Region of Cameroon. Sci J Public Health. 2015;3(4):525-537. doi: 10.11648/j.sjph.20150304.21

Copy | Download

@article{10.11648/j.sjph.20150304.21,
  author = {Kagning Tsinda Emmanuel and Fokunang Charles Ntungwen and Tchuenguem Fohouo Fernand-Nestor and Nwabo Kamdje Armel Herve and Nukenine Elias Nchinwan},
  title = {Epidemiology of Adenovirus and Genotypic Characteristics of Group A Rotavirus Among Diarrhoeic Children Aged 0 to 5 Years Old in the Ngaoundere Region of Cameroon},
  journal = {Science Journal of Public Health},
  volume = {3},
  number = {4},
  pages = {525-537},
  doi = {10.11648/j.sjph.20150304.21},
  url = {https://doi.org/10.11648/j.sjph.20150304.21},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjph.20150304.21},
  abstract = {In the context of recent introduction of a Rotavirus vaccine, little is known about the epidemiology of Rotavirus and Adenovirus gastroenteritis in Cameroon in general and in Ngaoundere specifically. The main objective of this study was to determine the frequency of Rotavirus and Adenovirus antigens as well as to characterise circulating Rotavirus strains in children with diarrhoea in Ngaoundere. A convenience sampling method was used and stool samples collected from patients consulting at the study sites from April to July 2014 were subjected to a qualitative immunochromatographic test to detect group antigens of group A Rotavirus and Adenovirus. Socio-demographic data were collected using a pre-tested questionnaire. Stool samples tested positive for Group A Rotavirus were genotyped by multiplex nested RT-PCR method. Logistic regression, Chi-square or Fisher’s exact tests were performed when appropriate. P-value < 0.05 at 95% confidence interval was considered statistically significant. Adenovirus antigen was detected in 26.4% (56) of children, whereas, group A Rotavirus was detected in 2.3% (5); the co-infection rate was 5.3%. Vomiting, fever, moderate dehydration, severe dehydration and the presence of polymorphonuclear cells in stool were significantly associated with the presence of Adenovirus antigen in stool. Group A Rotavirus was also found to circulate in Ngaoundere and the G1P[8] genotype showed indication of being the most represented. Adenovirus antigen was shown to be an important agent in childhood acute gastroenteritis in Ngaoundere and this study has been used as a baseline data for this infection in this region of Cameroon. The appropriate management of viral acute gastroenteritis necessitates the detection of Rotavirus and Adenovirus in clinical routine practice. Furthermore, Rotarix™ vaccination should be encouraged and the populations should be educated on hygiene practices.},
 year = {2015}
}

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TY  - JOUR
T1  - Epidemiology of Adenovirus and Genotypic Characteristics of Group A Rotavirus Among Diarrhoeic Children Aged 0 to 5 Years Old in the Ngaoundere Region of Cameroon
AU  - Kagning Tsinda Emmanuel
AU  - Fokunang Charles Ntungwen
AU  - Tchuenguem Fohouo Fernand-Nestor
AU  - Nwabo Kamdje Armel Herve
AU  - Nukenine Elias Nchinwan
Y1  - 2015/06/06
PY  - 2015
N1  - https://doi.org/10.11648/j.sjph.20150304.21
DO  - 10.11648/j.sjph.20150304.21
T2  - Science Journal of Public Health
JF  - Science Journal of Public Health
JO  - Science Journal of Public Health
SP  - 525
EP  - 537
PB  - Science Publishing Group
SN  - 2328-7950
UR  - https://doi.org/10.11648/j.sjph.20150304.21
AB  - In the context of recent introduction of a Rotavirus vaccine, little is known about the epidemiology of Rotavirus and Adenovirus gastroenteritis in Cameroon in general and in Ngaoundere specifically. The main objective of this study was to determine the frequency of Rotavirus and Adenovirus antigens as well as to characterise circulating Rotavirus strains in children with diarrhoea in Ngaoundere. A convenience sampling method was used and stool samples collected from patients consulting at the study sites from April to July 2014 were subjected to a qualitative immunochromatographic test to detect group antigens of group A Rotavirus and Adenovirus. Socio-demographic data were collected using a pre-tested questionnaire. Stool samples tested positive for Group A Rotavirus were genotyped by multiplex nested RT-PCR method. Logistic regression, Chi-square or Fisher’s exact tests were performed when appropriate. P-value < 0.05 at 95% confidence interval was considered statistically significant. Adenovirus antigen was detected in 26.4% (56) of children, whereas, group A Rotavirus was detected in 2.3% (5); the co-infection rate was 5.3%. Vomiting, fever, moderate dehydration, severe dehydration and the presence of polymorphonuclear cells in stool were significantly associated with the presence of Adenovirus antigen in stool. Group A Rotavirus was also found to circulate in Ngaoundere and the G1P[8] genotype showed indication of being the most represented. Adenovirus antigen was shown to be an important agent in childhood acute gastroenteritis in Ngaoundere and this study has been used as a baseline data for this infection in this region of Cameroon. The appropriate management of viral acute gastroenteritis necessitates the detection of Rotavirus and Adenovirus in clinical routine practice. Furthermore, Rotarix™ vaccination should be encouraged and the populations should be educated on hygiene practices.
VL  - 3
IS  - 4
ER  -

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