Electron spin resonance (ESR) spectroscopy studies on the reduction process of nitroxyl radicals were carried out for 1mM concentration of 14N-labeled nitroxyl radicals in 1 mM concentration of ascorbic acid as a function of time. The half life time and decay rate were estimated for 1mM concentration of 14N labeled nitroxyl radicals in 1 mM concentration of ascorbic acid. From the results, the increase in half life time and decrease in decay rate were calculated for TEMPONE compared with TEMPO and TEMPOL radicals, which indicates the higher stability of TEMPONE radical. The observed radical scavenging activity is also higher for TEMPONE radical. The ESR spectrum was also recorded for 1mM concentration of 14N-labeled nitroxyl radicals in pure water and the ESR parameters, line width, hyperfine coupling constant, g-factor, signal intensity ratio and rotational correlation time were obtained. These results indicate that the TEMPONE radical has narrowest line width and fast tumbling motion compared with TEMPO and TEMPOL. Therefore, this study reveals that the TEMPONE radical can act as a good redox sensitive spin probe for molecular imaging.
| Published in | European Journal of Biophysics (Volume 2, Issue 1) |
| DOI | 10.11648/j.ejb.20140201.11 |
| Page(s) | 1-6 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2014. Published by Science Publishing Group |
Electron Spin Resonance Spectroscopy, Nitroxyl Radicals, Ascorbic Acid, Half Life Time, Decay Rate
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APA Style
M. Kumara Dhas, A. Jawahar, A. Milton Franklin Benial. (2014). Electron Spin Resonance Spectroscopy Studies on Reduction Process of Nitroxyl Radicals used in Molecular Imaging. European Journal of Biophysics, 2(1), 1-6. https://doi.org/10.11648/j.ejb.20140201.11
ACS Style
M. Kumara Dhas; A. Jawahar; A. Milton Franklin Benial. Electron Spin Resonance Spectroscopy Studies on Reduction Process of Nitroxyl Radicals used in Molecular Imaging. Eur. J. Biophys. 2014, 2(1), 1-6. doi: 10.11648/j.ejb.20140201.11
AMA Style
M. Kumara Dhas, A. Jawahar, A. Milton Franklin Benial. Electron Spin Resonance Spectroscopy Studies on Reduction Process of Nitroxyl Radicals used in Molecular Imaging. Eur J Biophys. 2014;2(1):1-6. doi: 10.11648/j.ejb.20140201.11
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Download@article{10.11648/j.ejb.20140201.11,
author = {M. Kumara Dhas and A. Jawahar and A. Milton Franklin Benial},
title = {Electron Spin Resonance Spectroscopy Studies on Reduction Process of Nitroxyl Radicals used in Molecular Imaging},
journal = {European Journal of Biophysics},
volume = {2},
number = {1},
pages = {1-6},
doi = {10.11648/j.ejb.20140201.11},
url = {https://doi.org/10.11648/j.ejb.20140201.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ejb.20140201.11},
abstract = {Electron spin resonance (ESR) spectroscopy studies on the reduction process of nitroxyl radicals were carried out for 1mM concentration of 14N-labeled nitroxyl radicals in 1 mM concentration of ascorbic acid as a function of time. The half life time and decay rate were estimated for 1mM concentration of 14N labeled nitroxyl radicals in 1 mM concentration of ascorbic acid. From the results, the increase in half life time and decrease in decay rate were calculated for TEMPONE compared with TEMPO and TEMPOL radicals, which indicates the higher stability of TEMPONE radical. The observed radical scavenging activity is also higher for TEMPONE radical. The ESR spectrum was also recorded for 1mM concentration of 14N-labeled nitroxyl radicals in pure water and the ESR parameters, line width, hyperfine coupling constant, g-factor, signal intensity ratio and rotational correlation time were obtained. These results indicate that the TEMPONE radical has narrowest line width and fast tumbling motion compared with TEMPO and TEMPOL. Therefore, this study reveals that the TEMPONE radical can act as a good redox sensitive spin probe for molecular imaging.},
year = {2014}
}
TY - JOUR T1 - Electron Spin Resonance Spectroscopy Studies on Reduction Process of Nitroxyl Radicals used in Molecular Imaging AU - M. Kumara Dhas AU - A. Jawahar AU - A. Milton Franklin Benial Y1 - 2014/02/28 PY - 2014 N1 - https://doi.org/10.11648/j.ejb.20140201.11 DO - 10.11648/j.ejb.20140201.11 T2 - European Journal of Biophysics JF - European Journal of Biophysics JO - European Journal of Biophysics SP - 1 EP - 6 PB - Science Publishing Group SN - 2329-1737 UR - https://doi.org/10.11648/j.ejb.20140201.11 AB - Electron spin resonance (ESR) spectroscopy studies on the reduction process of nitroxyl radicals were carried out for 1mM concentration of 14N-labeled nitroxyl radicals in 1 mM concentration of ascorbic acid as a function of time. The half life time and decay rate were estimated for 1mM concentration of 14N labeled nitroxyl radicals in 1 mM concentration of ascorbic acid. From the results, the increase in half life time and decrease in decay rate were calculated for TEMPONE compared with TEMPO and TEMPOL radicals, which indicates the higher stability of TEMPONE radical. The observed radical scavenging activity is also higher for TEMPONE radical. The ESR spectrum was also recorded for 1mM concentration of 14N-labeled nitroxyl radicals in pure water and the ESR parameters, line width, hyperfine coupling constant, g-factor, signal intensity ratio and rotational correlation time were obtained. These results indicate that the TEMPONE radical has narrowest line width and fast tumbling motion compared with TEMPO and TEMPOL. Therefore, this study reveals that the TEMPONE radical can act as a good redox sensitive spin probe for molecular imaging. VL - 2 IS - 1 ER -
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