Recent breakthrough studies gave a considerable interest on the pathogenesis of periodontal diseases, particularly, the critical role of cytokines. Cytokines have dual roles; they could be mediators of host defense against the microbial challenge, or mediators for periodontal tissue alteration. Osteopontin (OPN) is a glycosylated phosphoprotein, it functions as a Th 1 (T – helper 1) cytokine, promotes cell-mediated immune responses and plays a role in chronic inflammatory diseases. To assess OPN expression in dentogingival junction (DGJ) in chronic periodontitis, ten 13–16 months old, beagle dogs were used. Animals were distributed randomly into two equal groups: Group I: animals had healthy periodontium. Group II: chronic periodontitis was induced in animals. Four weeks after induction, dogs were sacrificed and specimens were prepared for immunohistochemical staining using OPN monoclonal antibody. Intensity of OPN reactions was recorded by image J and t-test was used to compare between the two groups. Healthy periodontium showed a mild OPN reaction. While, in chronic periodontitis, moderate to intense extracellular reaction was seen in DGJ. Also, sporadic nuclear OPN reactions of inflammatory cells were noticed in chronic periodontitis. T-test revealed a significant difference between normal and chronic periodontitis groups. In conclusion, OPN may play a role in chronic inflammation and can be considered as a biomarker of periodontal disease progression.
| Published in | American Journal of Life Sciences (Volume 2, Issue 6) |
| DOI | 10.11648/j.ajls.20140206.21 |
| Page(s) | 400-405 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2015. Published by Science Publishing Group |
OPN, Chronic Periodontitis, DGJ, Periodontium, Dogs
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APA Style
Olfat Mohamed Gab Allah, Mona Assem Abd-Elmotelb, Reda Gaber Saleh. (2015). Expression of Bone Matrix Protein (Osteopontin) in Dentogingival Junction in Periodontal Disease. American Journal of Life Sciences, 2(6), 400-405. https://doi.org/10.11648/j.ajls.20140206.21
ACS Style
Olfat Mohamed Gab Allah; Mona Assem Abd-Elmotelb; Reda Gaber Saleh. Expression of Bone Matrix Protein (Osteopontin) in Dentogingival Junction in Periodontal Disease. Am. J. Life Sci. 2015, 2(6), 400-405. doi: 10.11648/j.ajls.20140206.21
AMA Style
Olfat Mohamed Gab Allah, Mona Assem Abd-Elmotelb, Reda Gaber Saleh. Expression of Bone Matrix Protein (Osteopontin) in Dentogingival Junction in Periodontal Disease. Am J Life Sci. 2015;2(6):400-405. doi: 10.11648/j.ajls.20140206.21
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Download@article{10.11648/j.ajls.20140206.21,
author = {Olfat Mohamed Gab Allah and Mona Assem Abd-Elmotelb and Reda Gaber Saleh},
title = {Expression of Bone Matrix Protein (Osteopontin) in Dentogingival Junction in Periodontal Disease},
journal = {American Journal of Life Sciences},
volume = {2},
number = {6},
pages = {400-405},
doi = {10.11648/j.ajls.20140206.21},
url = {https://doi.org/10.11648/j.ajls.20140206.21},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20140206.21},
abstract = {Recent breakthrough studies gave a considerable interest on the pathogenesis of periodontal diseases, particularly, the critical role of cytokines. Cytokines have dual roles; they could be mediators of host defense against the microbial challenge, or mediators for periodontal tissue alteration. Osteopontin (OPN) is a glycosylated phosphoprotein, it functions as a Th 1 (T – helper 1) cytokine, promotes cell-mediated immune responses and plays a role in chronic inflammatory diseases. To assess OPN expression in dentogingival junction (DGJ) in chronic periodontitis, ten 13–16 months old, beagle dogs were used. Animals were distributed randomly into two equal groups: Group I: animals had healthy periodontium. Group II: chronic periodontitis was induced in animals. Four weeks after induction, dogs were sacrificed and specimens were prepared for immunohistochemical staining using OPN monoclonal antibody. Intensity of OPN reactions was recorded by image J and t-test was used to compare between the two groups. Healthy periodontium showed a mild OPN reaction. While, in chronic periodontitis, moderate to intense extracellular reaction was seen in DGJ. Also, sporadic nuclear OPN reactions of inflammatory cells were noticed in chronic periodontitis. T-test revealed a significant difference between normal and chronic periodontitis groups. In conclusion, OPN may play a role in chronic inflammation and can be considered as a biomarker of periodontal disease progression.},
year = {2015}
}
TY - JOUR T1 - Expression of Bone Matrix Protein (Osteopontin) in Dentogingival Junction in Periodontal Disease AU - Olfat Mohamed Gab Allah AU - Mona Assem Abd-Elmotelb AU - Reda Gaber Saleh Y1 - 2015/01/06 PY - 2015 N1 - https://doi.org/10.11648/j.ajls.20140206.21 DO - 10.11648/j.ajls.20140206.21 T2 - American Journal of Life Sciences JF - American Journal of Life Sciences JO - American Journal of Life Sciences SP - 400 EP - 405 PB - Science Publishing Group SN - 2328-5737 UR - https://doi.org/10.11648/j.ajls.20140206.21 AB - Recent breakthrough studies gave a considerable interest on the pathogenesis of periodontal diseases, particularly, the critical role of cytokines. Cytokines have dual roles; they could be mediators of host defense against the microbial challenge, or mediators for periodontal tissue alteration. Osteopontin (OPN) is a glycosylated phosphoprotein, it functions as a Th 1 (T – helper 1) cytokine, promotes cell-mediated immune responses and plays a role in chronic inflammatory diseases. To assess OPN expression in dentogingival junction (DGJ) in chronic periodontitis, ten 13–16 months old, beagle dogs were used. Animals were distributed randomly into two equal groups: Group I: animals had healthy periodontium. Group II: chronic periodontitis was induced in animals. Four weeks after induction, dogs were sacrificed and specimens were prepared for immunohistochemical staining using OPN monoclonal antibody. Intensity of OPN reactions was recorded by image J and t-test was used to compare between the two groups. Healthy periodontium showed a mild OPN reaction. While, in chronic periodontitis, moderate to intense extracellular reaction was seen in DGJ. Also, sporadic nuclear OPN reactions of inflammatory cells were noticed in chronic periodontitis. T-test revealed a significant difference between normal and chronic periodontitis groups. In conclusion, OPN may play a role in chronic inflammation and can be considered as a biomarker of periodontal disease progression. VL - 2 IS - 6 ER -
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