Staphylococcus aureus strains are responsible for a range of acute to chronic infections in humans and other animals. There is scanty information about the genetic background of S. aureus strains in Rivers State, Nigeria. The aim of this study was to determine the occurrence of MRSA among S. aureus isolates as well as detect the presence of mecA gene among methicillin-resistant Staphylococcus aureus isolates in Port Harcourt, Nigeria. Two hundred and five (205) non duplicate Staphylococcus aureus previously isolated from human sources were randomly collected from three health facilities- University of Port Harcourt Teaching Hospital, Braithwaite Memorial Specialist Hospital and De-Integrated Laboratories- all located in Port Harcourt, Nigeria, for this study from August, 2012 to July, 2013. Isolates were grouped as hospital in-patient (Hospital-acquired – Nosocomial; n = 76) and out-patient cases (community-acquired; n = 129). Isolates were reconfirmed following standard laboratory protocols and stored in duplicate - one set at +4°C (for phenotypic detection of MRSA) and another set at -70°C for molecular analysis. Using the disk diffusion method, detection of MRSA was carried out with 1μg of oxacillin (OXOID) placed on Mueller-Hinton agar with 4% NaCl supplementation). Molecular Analyses were carried out on all ORSA strains as follows- Bacterial genomic DNA extraction and PCR amplification for detection of 16S rRNA and mecA genes. Amplified products were analyzed using 2.0% agarose gel electrophoresis and subsequently visualized on a UV trans-illuminator. About twelve percent (12.2%) of the 205 Staphylococcus aureus studied were resistant to oxacillin. MRSA detection was significantly higher in in-patient isolates (23.7% of 76) than out-patient (5.4% of 129) S. aureus (p = 0.00031). Urine samples accounted for majority of the isolates (52 of 205) but MRSA detection was highest in Wound swabs (9 of 48 isolates. Of the 25 MRSA, mecA gene was detected in 17, being significantly higher in in-patient MRSA (14) than out-patient MRSA (3) (p<0-05). This study has established the presence of the methicillin resistance encoding gene- mecA, among MRSA isolates in Port Harcourt and that this gene is largely responsible for the MRSA phenotype. Study further establishes that these MRSA are more frequent in the Hospital environment. Further studies on molecular epidemiology of S. aureus are recommended in this region. Improved infection control measures in the healthcare facilities as well as sustained surveillance of methicillin-resistant S. aureus in this region are also advocated.
| Published in | American Journal of Biomedical and Life Sciences (Volume 4, Issue 3) |
| DOI | 10.11648/j.ajbls.20160403.14 |
| Page(s) | 41-48 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2016. Published by Science Publishing Group |
Staphylococcus aureus, MRSA, mecA Gene
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APA Style
Easter Godwin Nwokah, Samuel Douglas Abbey, Confidence Kinikanwo Wachukwu. (2016). mecA Gene Profile of Methicillin-Resistant Staphylococcus aureus Isolates from Clinical Sources in Port Harcourt, Nigeria. American Journal of Biomedical and Life Sciences, 4(3), 41-48. https://doi.org/10.11648/j.ajbls.20160403.14
ACS Style
Easter Godwin Nwokah; Samuel Douglas Abbey; Confidence Kinikanwo Wachukwu. mecA Gene Profile of Methicillin-Resistant Staphylococcus aureus Isolates from Clinical Sources in Port Harcourt, Nigeria. Am. J. Biomed. Life Sci. 2016, 4(3), 41-48. doi: 10.11648/j.ajbls.20160403.14
AMA Style
Easter Godwin Nwokah, Samuel Douglas Abbey, Confidence Kinikanwo Wachukwu. mecA Gene Profile of Methicillin-Resistant Staphylococcus aureus Isolates from Clinical Sources in Port Harcourt, Nigeria. Am J Biomed Life Sci. 2016;4(3):41-48. doi: 10.11648/j.ajbls.20160403.14
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Download@article{10.11648/j.ajbls.20160403.14,
author = {Easter Godwin Nwokah and Samuel Douglas Abbey and Confidence Kinikanwo Wachukwu},
title = {mecA Gene Profile of Methicillin-Resistant Staphylococcus aureus Isolates from Clinical Sources in Port Harcourt, Nigeria},
journal = {American Journal of Biomedical and Life Sciences},
volume = {4},
number = {3},
pages = {41-48},
doi = {10.11648/j.ajbls.20160403.14},
url = {https://doi.org/10.11648/j.ajbls.20160403.14},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20160403.14},
abstract = {Staphylococcus aureus strains are responsible for a range of acute to chronic infections in humans and other animals. There is scanty information about the genetic background of S. aureus strains in Rivers State, Nigeria. The aim of this study was to determine the occurrence of MRSA among S. aureus isolates as well as detect the presence of mecA gene among methicillin-resistant Staphylococcus aureus isolates in Port Harcourt, Nigeria. Two hundred and five (205) non duplicate Staphylococcus aureus previously isolated from human sources were randomly collected from three health facilities- University of Port Harcourt Teaching Hospital, Braithwaite Memorial Specialist Hospital and De-Integrated Laboratories- all located in Port Harcourt, Nigeria, for this study from August, 2012 to July, 2013. Isolates were grouped as hospital in-patient (Hospital-acquired – Nosocomial; n = 76) and out-patient cases (community-acquired; n = 129). Isolates were reconfirmed following standard laboratory protocols and stored in duplicate - one set at +4°C (for phenotypic detection of MRSA) and another set at -70°C for molecular analysis. Using the disk diffusion method, detection of MRSA was carried out with 1μg of oxacillin (OXOID) placed on Mueller-Hinton agar with 4% NaCl supplementation). Molecular Analyses were carried out on all ORSA strains as follows- Bacterial genomic DNA extraction and PCR amplification for detection of 16S rRNA and mecA genes. Amplified products were analyzed using 2.0% agarose gel electrophoresis and subsequently visualized on a UV trans-illuminator. About twelve percent (12.2%) of the 205 Staphylococcus aureus studied were resistant to oxacillin. MRSA detection was significantly higher in in-patient isolates (23.7% of 76) than out-patient (5.4% of 129) S. aureus (p = 0.00031). Urine samples accounted for majority of the isolates (52 of 205) but MRSA detection was highest in Wound swabs (9 of 48 isolates. Of the 25 MRSA, mecA gene was detected in 17, being significantly higher in in-patient MRSA (14) than out-patient MRSA (3) (pmecA, among MRSA isolates in Port Harcourt and that this gene is largely responsible for the MRSA phenotype. Study further establishes that these MRSA are more frequent in the Hospital environment. Further studies on molecular epidemiology of S. aureus are recommended in this region. Improved infection control measures in the healthcare facilities as well as sustained surveillance of methicillin-resistant S. aureus in this region are also advocated.},
year = {2016}
}
TY - JOUR T1 - mecA Gene Profile of Methicillin-Resistant Staphylococcus aureus Isolates from Clinical Sources in Port Harcourt, Nigeria AU - Easter Godwin Nwokah AU - Samuel Douglas Abbey AU - Confidence Kinikanwo Wachukwu Y1 - 2016/05/13 PY - 2016 N1 - https://doi.org/10.11648/j.ajbls.20160403.14 DO - 10.11648/j.ajbls.20160403.14 T2 - American Journal of Biomedical and Life Sciences JF - American Journal of Biomedical and Life Sciences JO - American Journal of Biomedical and Life Sciences SP - 41 EP - 48 PB - Science Publishing Group SN - 2330-880X UR - https://doi.org/10.11648/j.ajbls.20160403.14 AB - Staphylococcus aureus strains are responsible for a range of acute to chronic infections in humans and other animals. There is scanty information about the genetic background of S. aureus strains in Rivers State, Nigeria. The aim of this study was to determine the occurrence of MRSA among S. aureus isolates as well as detect the presence of mecA gene among methicillin-resistant Staphylococcus aureus isolates in Port Harcourt, Nigeria. Two hundred and five (205) non duplicate Staphylococcus aureus previously isolated from human sources were randomly collected from three health facilities- University of Port Harcourt Teaching Hospital, Braithwaite Memorial Specialist Hospital and De-Integrated Laboratories- all located in Port Harcourt, Nigeria, for this study from August, 2012 to July, 2013. Isolates were grouped as hospital in-patient (Hospital-acquired – Nosocomial; n = 76) and out-patient cases (community-acquired; n = 129). Isolates were reconfirmed following standard laboratory protocols and stored in duplicate - one set at +4°C (for phenotypic detection of MRSA) and another set at -70°C for molecular analysis. Using the disk diffusion method, detection of MRSA was carried out with 1μg of oxacillin (OXOID) placed on Mueller-Hinton agar with 4% NaCl supplementation). Molecular Analyses were carried out on all ORSA strains as follows- Bacterial genomic DNA extraction and PCR amplification for detection of 16S rRNA and mecA genes. Amplified products were analyzed using 2.0% agarose gel electrophoresis and subsequently visualized on a UV trans-illuminator. About twelve percent (12.2%) of the 205 Staphylococcus aureus studied were resistant to oxacillin. MRSA detection was significantly higher in in-patient isolates (23.7% of 76) than out-patient (5.4% of 129) S. aureus (p = 0.00031). Urine samples accounted for majority of the isolates (52 of 205) but MRSA detection was highest in Wound swabs (9 of 48 isolates. Of the 25 MRSA, mecA gene was detected in 17, being significantly higher in in-patient MRSA (14) than out-patient MRSA (3) (pmecA, among MRSA isolates in Port Harcourt and that this gene is largely responsible for the MRSA phenotype. Study further establishes that these MRSA are more frequent in the Hospital environment. Further studies on molecular epidemiology of S. aureus are recommended in this region. Improved infection control measures in the healthcare facilities as well as sustained surveillance of methicillin-resistant S. aureus in this region are also advocated. VL - 4 IS - 3 ER -
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