UV Absorbance of Aqueous DNA

Pivovarenko Yuri Vadimovich

doi:doi:10.11648/j.ejb.20150303.11

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UV Absorbance of Aqueous DNA

Pivovarenko Yuri Vadimovich

Published in European Journal of Biophysics (Volume 3, Issue 3)

Received: 7 May 2015 Accepted: 13 May 2015 Published: 1 June 2015

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Abstract

The UV absorption spectra of water with different electric charge (potential) were obtained. It was shown that UV absorption spectra of water with negative electric charge (potential) have the sharp peaks with maximum in the range 190 – 200 nm and UV absorption spectra of water with positive electric charge (potential) have the wide peaks with maximum in the range 200 – 220 nm. It was asked to explain this absorption. It was also established that UV absorption spectra of water solutions of surface inactive substances have sharp peaks with a maximum in the range 190 – 200 nm and UV absorption spectra of solutions of surface active substances have the wide peaks with a maximum in the range 200 – 220 nm. The UV absorption spectra of DNA solutions, which were prepared on the water with different electric charge (potential), were obtained. It was shown that these spectra are dependent on the electric charge (potential) of water used. It was proposed that UV absorption spectra of aqueous DNA reflect mostly the spectral properties of charged water or charged cuvette.

Published in	European Journal of Biophysics (Volume 3, Issue 3)
DOI	10.11648/j.ejb.20150303.11
Page(s)	19-22
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

UV Absorption Spectra, Electric Charge, Electric Potential, DNA

References

[1]	Shabarova Z.A. and Bogdanov A.A. (1978) Chemistry of Nucleic Acids and their Components, Moscow: Chemistry.
[2]	Nekrasov B.V. (1974) Bases of General Chemistry 1, Moscow: Chemistry.
[3]	Fridrichsberg D.A. (1974) Course of Colloid Chemistry Leningrad: Chemistry.
[4]	Stark, K.B.; Gallas, J.M.; Zajac G.W.; Golab, J.T.; Gidanian, S.; McIntire, T.; Farmer, P.J. (2005) The effect of stacking and redox state on optical absorption spectra of melanins- a comparison of theoretical and experimental results. J. Phys. Chem. B., 109, 1970-1977.
[5]	Israel H., and Knopp R. (1962) Zum Problem der Ladungbildung beim Verdaumpfen, Arch. Meteorol., Geophys. and Bioklimatol. A13, 199-206.
[6]	Latham J., and Stow C.D. (1965) Electrification of evaporation of ice crystals. Materials of Intern. Conf. in Tokyo, 352–356.
[7]	Krasnogorskaja N.V. (1984) Electromagnetic fields in the earth’s atmosphere and their biological significance 1, Moscow: Nauka.
[8]	Pivovarenko, Y. (2015) A Charge Distribution in the Earth’s Atmosphere American Journal of Physics and Applications. 3 (3), 67-68.
[9]	Doshi, R., Day, P. J. R., and Tirelli, N. (2009) Dissolved oxygen alteration of the spectrophotometric analysis and quantification of nucleic acid solutions. Biochemical Society Transactions, 37(2), 466–470.
[10]	Doshi, R., Day, P.J.R., Carampin, P., Blanch, E., Statford, I.J. and Tirelli, N. (2010) Spectrophotometric analysis of nucleic acids: oxygenation-dependant hyperchromism of DNA. Anal. Bioanal. Chem., 396, 2331–2339.
[11]	Pivovarenko, Y. V. (2014) Hypochromism degassed solutions of DNA Modern high technologies. 3, 147-150.
[12]	Mergny, J.-L., Li, J., Lacroix, L., Amrane, S. and Chaires, J. B. (2005) Thermal difference spectra: a specific signature for ucleic acid structures. Nucleic Acids Res., 33(16), 1–6.

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APA Style

Pivovarenko Yuri Vadimovich. (2015). UV Absorbance of Aqueous DNA. European Journal of Biophysics, 3(3), 19-22. https://doi.org/10.11648/j.ejb.20150303.11

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ACS Style

Pivovarenko Yuri Vadimovich. UV Absorbance of Aqueous DNA. Eur. J. Biophys. 2015, 3(3), 19-22. doi: 10.11648/j.ejb.20150303.11

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AMA Style

Pivovarenko Yuri Vadimovich. UV Absorbance of Aqueous DNA. Eur J Biophys. 2015;3(3):19-22. doi: 10.11648/j.ejb.20150303.11

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@article{10.11648/j.ejb.20150303.11,
  author = {Pivovarenko Yuri Vadimovich},
  title = {UV Absorbance of Aqueous DNA},
  journal = {European Journal of Biophysics},
  volume = {3},
  number = {3},
  pages = {19-22},
  doi = {10.11648/j.ejb.20150303.11},
  url = {https://doi.org/10.11648/j.ejb.20150303.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ejb.20150303.11},
  abstract = {The UV absorption spectra of water with different electric charge (potential) were obtained. It was shown that UV absorption spectra of water with negative electric charge (potential) have the sharp peaks with maximum in the range 190 – 200 nm and UV absorption spectra of water with positive electric charge (potential) have the wide peaks with maximum in the range 200 – 220 nm. It was asked to explain this absorption. It was also established that UV absorption spectra of water solutions of surface inactive substances have sharp peaks with a maximum in the range 190 – 200 nm and UV absorption spectra of solutions of surface active substances have the wide peaks with a maximum in the range 200 – 220 nm. The UV absorption spectra of DNA solutions, which were prepared on the water with different electric charge (potential), were obtained. It was shown that these spectra are dependent on the electric charge (potential) of water used. It was proposed that UV absorption spectra of aqueous DNA reflect mostly the spectral properties of charged water or charged cuvette.},
 year = {2015}
}

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TY  - JOUR
T1  - UV Absorbance of Aqueous DNA
AU  - Pivovarenko Yuri Vadimovich
Y1  - 2015/06/01
PY  - 2015
N1  - https://doi.org/10.11648/j.ejb.20150303.11
DO  - 10.11648/j.ejb.20150303.11
T2  - European Journal of Biophysics
JF  - European Journal of Biophysics
JO  - European Journal of Biophysics
SP  - 19
EP  - 22
PB  - Science Publishing Group
SN  - 2329-1737
UR  - https://doi.org/10.11648/j.ejb.20150303.11
AB  - The UV absorption spectra of water with different electric charge (potential) were obtained. It was shown that UV absorption spectra of water with negative electric charge (potential) have the sharp peaks with maximum in the range 190 – 200 nm and UV absorption spectra of water with positive electric charge (potential) have the wide peaks with maximum in the range 200 – 220 nm. It was asked to explain this absorption. It was also established that UV absorption spectra of water solutions of surface inactive substances have sharp peaks with a maximum in the range 190 – 200 nm and UV absorption spectra of solutions of surface active substances have the wide peaks with a maximum in the range 200 – 220 nm. The UV absorption spectra of DNA solutions, which were prepared on the water with different electric charge (potential), were obtained. It was shown that these spectra are dependent on the electric charge (potential) of water used. It was proposed that UV absorption spectra of aqueous DNA reflect mostly the spectral properties of charged water or charged cuvette.
VL  - 3
IS  - 3
ER  -

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Author Information

Pivovarenko Yuri Vadimovich

Research and Training Center ‘Physical and Chemical Materials Science’ under Kyiv Taras Shevchenko University and NAS of Ukraine, Kiev, Ukraine

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APA Style

Pivovarenko Yuri Vadimovich. (2015). UV Absorbance of Aqueous DNA. European Journal of Biophysics, 3(3), 19-22. https://doi.org/10.11648/j.ejb.20150303.11

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ACS Style

Pivovarenko Yuri Vadimovich. UV Absorbance of Aqueous DNA. Eur. J. Biophys. 2015, 3(3), 19-22. doi: 10.11648/j.ejb.20150303.11

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AMA Style

Pivovarenko Yuri Vadimovich. UV Absorbance of Aqueous DNA. Eur J Biophys. 2015;3(3):19-22. doi: 10.11648/j.ejb.20150303.11

Copy | Download

@article{10.11648/j.ejb.20150303.11,
  author = {Pivovarenko Yuri Vadimovich},
  title = {UV Absorbance of Aqueous DNA},
  journal = {European Journal of Biophysics},
  volume = {3},
  number = {3},
  pages = {19-22},
  doi = {10.11648/j.ejb.20150303.11},
  url = {https://doi.org/10.11648/j.ejb.20150303.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ejb.20150303.11},
  abstract = {The UV absorption spectra of water with different electric charge (potential) were obtained. It was shown that UV absorption spectra of water with negative electric charge (potential) have the sharp peaks with maximum in the range 190 – 200 nm and UV absorption spectra of water with positive electric charge (potential) have the wide peaks with maximum in the range 200 – 220 nm. It was asked to explain this absorption. It was also established that UV absorption spectra of water solutions of surface inactive substances have sharp peaks with a maximum in the range 190 – 200 nm and UV absorption spectra of solutions of surface active substances have the wide peaks with a maximum in the range 200 – 220 nm. The UV absorption spectra of DNA solutions, which were prepared on the water with different electric charge (potential), were obtained. It was shown that these spectra are dependent on the electric charge (potential) of water used. It was proposed that UV absorption spectra of aqueous DNA reflect mostly the spectral properties of charged water or charged cuvette.},
 year = {2015}
}

Copy | Download

TY  - JOUR
T1  - UV Absorbance of Aqueous DNA
AU  - Pivovarenko Yuri Vadimovich
Y1  - 2015/06/01
PY  - 2015
N1  - https://doi.org/10.11648/j.ejb.20150303.11
DO  - 10.11648/j.ejb.20150303.11
T2  - European Journal of Biophysics
JF  - European Journal of Biophysics
JO  - European Journal of Biophysics
SP  - 19
EP  - 22
PB  - Science Publishing Group
SN  - 2329-1737
UR  - https://doi.org/10.11648/j.ejb.20150303.11
AB  - The UV absorption spectra of water with different electric charge (potential) were obtained. It was shown that UV absorption spectra of water with negative electric charge (potential) have the sharp peaks with maximum in the range 190 – 200 nm and UV absorption spectra of water with positive electric charge (potential) have the wide peaks with maximum in the range 200 – 220 nm. It was asked to explain this absorption. It was also established that UV absorption spectra of water solutions of surface inactive substances have sharp peaks with a maximum in the range 190 – 200 nm and UV absorption spectra of solutions of surface active substances have the wide peaks with a maximum in the range 200 – 220 nm. The UV absorption spectra of DNA solutions, which were prepared on the water with different electric charge (potential), were obtained. It was shown that these spectra are dependent on the electric charge (potential) of water used. It was proposed that UV absorption spectra of aqueous DNA reflect mostly the spectral properties of charged water or charged cuvette.
VL  - 3
IS  - 3
ER  -

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