Antibiotic Susceptibility and Plasmid Profile of Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli Isolated from Wound Patients in Abakaliki Metropolis, Ebonyi State

Nworie O.; Nnachi A. U.; Ukaegbu C. O; Alo M. N.; Ekuma U. O.; Ogueji E. O.

doi:doi:10.11648/j.bio.20130106.11

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Antibiotic Susceptibility and Plasmid Profile of Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli Isolated from Wound Patients in Abakaliki Metropolis, Ebonyi State

Nworie O., Nnachi A. U., Ukaegbu C. O, Alo M. N., Ekuma U. O., Ogueji E. O.

Published in American Journal of Bioscience and Bioengineering (Volume 1, Issue 6)

Received: 7 September 2013 Published: 20 October 2013

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Abstract

Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli has become increasingly recognized as an emerging opportunistic pathogen of clinical importance. A total of 18 isolates comprising of 10 (55.6%) S. aureus, 6 (33.3%) P. aeruginosa and 2 (11.1%) E. coli were recovered from 15 pus samples of wound patients attending Federal Teaching Hospital Abakaliki I (FETHA I) and Federal Teaching Hospital Abakaliki II (FETHA II). All the isolates were subjected to antibiotic susceptibility testing using Kirby-Bauer disk diffusion method on Mueller-Hinton agar. S. aureus showed the highest susceptibility percentage of 80.0% to ciprofloxacin, followed by 60.0% to augmentin and 50.0% to streptomycin, while the highest resistance percentage was obtained with lincocin (100.0%), followed by ampiclox (80.0%). P. aeruginosa showed the highest susceptibility percentage of 83.3% to ciprofloxacin, followed by 66.6% to streptomycin and gentamycin (66.6%), while the highest resistance percentage was obtained with streptomycin (33.3%) and gentamycin (33.3%). E. coli showed the highest susceptibility percentage to gentamycin and streptomycin with 100% activity. The antibiotics with reasonable resistant profile was observed in 10 isolates (5 S. aureus, 5 P. aeruginosa and 1 E. coli) with isolate code S1, S2, S3, S4, S5, Ps1, Ps2, Ps3, Ps4, Ps5 and Ec1 which showed resistance to atleast 5 antibiotics, hence this isolates were subjected to plasmid profile analysis. Only three isolates (S1, S4 and Ec1) showed the presence of plasmids within the range of 1.8 kbp to 10.4 kbp. Hence antibiotic resistance of an organism does not always confer the presence of plasmid.

Published in	American Journal of Bioscience and Bioengineering (Volume 1, Issue 6)
DOI	10.11648/j.bio.20130106.11
Page(s)	75-82
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2013. Published by Science Publishing Group

Keywords

Antibiotics, Plasmid, S. aureus, P. aeruginosa, E. coli, Wound Infection, Abakaliki

References

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Nworie O., Nnachi A. U., Ukaegbu C. O, Alo M. N., Ekuma U. O., et al. (2013). Antibiotic Susceptibility and Plasmid Profile of Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli Isolated from Wound Patients in Abakaliki Metropolis, Ebonyi State. American Journal of Bioscience and Bioengineering, 1(6), 75-82. https://doi.org/10.11648/j.bio.20130106.11

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Nworie O.; Nnachi A. U.; Ukaegbu C. O; Alo M. N.; Ekuma U. O., et al. Antibiotic Susceptibility and Plasmid Profile of Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli Isolated from Wound Patients in Abakaliki Metropolis, Ebonyi State. Am. J. BioSci. Bioeng. 2013, 1(6), 75-82. doi: 10.11648/j.bio.20130106.11

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Nworie O., Nnachi A. U., Ukaegbu C. O, Alo M. N., Ekuma U. O., et al. Antibiotic Susceptibility and Plasmid Profile of Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli Isolated from Wound Patients in Abakaliki Metropolis, Ebonyi State. Am J BioSci Bioeng. 2013;1(6):75-82. doi: 10.11648/j.bio.20130106.11

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@article{10.11648/j.bio.20130106.11,
  author = {Nworie O. and Nnachi A. U. and Ukaegbu C. O and Alo M. N. and Ekuma U. O. and Ogueji E. O.},
  title = {Antibiotic Susceptibility and Plasmid Profile of Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli Isolated from Wound Patients in Abakaliki Metropolis, Ebonyi State},
  journal = {American Journal of Bioscience and Bioengineering},
  volume = {1},
  number = {6},
  pages = {75-82},
  doi = {10.11648/j.bio.20130106.11},
  url = {https://doi.org/10.11648/j.bio.20130106.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20130106.11},
  abstract = {Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli has become increasingly recognized as an emerging opportunistic pathogen of clinical importance. A total of 18 isolates comprising of 10 (55.6%) S. aureus, 6 (33.3%) P. aeruginosa and 2 (11.1%) E. coli were recovered from 15 pus samples of wound patients attending Federal Teaching Hospital Abakaliki I (FETHA I) and Federal Teaching Hospital Abakaliki II (FETHA II). All the isolates were subjected to antibiotic susceptibility testing using Kirby-Bauer disk diffusion method on Mueller-Hinton agar. S. aureus showed the highest susceptibility percentage of 80.0% to ciprofloxacin, followed by 60.0% to augmentin and 50.0% to streptomycin, while the highest resistance percentage was obtained with lincocin (100.0%), followed by ampiclox (80.0%). P. aeruginosa showed the highest susceptibility percentage of 83.3% to ciprofloxacin, followed by 66.6% to streptomycin and gentamycin (66.6%), while the highest resistance percentage was obtained with streptomycin (33.3%) and gentamycin (33.3%). E. coli showed the highest susceptibility percentage to gentamycin and streptomycin with 100% activity. The antibiotics with reasonable resistant profile was observed in 10 isolates (5 S. aureus, 5 P. aeruginosa and 1 E. coli) with isolate code S1, S2, S3, S4, S5, Ps1, Ps2, Ps3, Ps4, Ps5 and Ec1 which showed resistance to atleast 5 antibiotics, hence this isolates were subjected to plasmid profile analysis. Only three isolates (S1, S4 and Ec1) showed the presence of plasmids within the range of 1.8 kbp to 10.4 kbp. Hence antibiotic resistance of an organism does not always confer the presence of plasmid.},
 year = {2013}
}

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TY - JOUR
T1 - Antibiotic Susceptibility and Plasmid Profile of Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli Isolated from Wound Patients in Abakaliki Metropolis, Ebonyi State
AU - Nworie O.
AU - Nnachi A. U.
AU - Ukaegbu C. O
AU - Alo M. N.
AU - Ekuma U. O.
AU - Ogueji E. O.
Y1 - 2013/10/20
PY - 2013
N1 - https://doi.org/10.11648/j.bio.20130106.11
DO - 10.11648/j.bio.20130106.11
T2 - American Journal of Bioscience and Bioengineering
JF - American Journal of Bioscience and Bioengineering
JO - American Journal of Bioscience and Bioengineering
SP - 75
EP - 82
PB - Science Publishing Group
SN - 2328-5893
UR - https://doi.org/10.11648/j.bio.20130106.11
AB - Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli has become increasingly recognized as an emerging opportunistic pathogen of clinical importance. A total of 18 isolates comprising of 10 (55.6%) S. aureus, 6 (33.3%) P. aeruginosa and 2 (11.1%) E. coli were recovered from 15 pus samples of wound patients attending Federal Teaching Hospital Abakaliki I (FETHA I) and Federal Teaching Hospital Abakaliki II (FETHA II). All the isolates were subjected to antibiotic susceptibility testing using Kirby-Bauer disk diffusion method on Mueller-Hinton agar. S. aureus showed the highest susceptibility percentage of 80.0% to ciprofloxacin, followed by 60.0% to augmentin and 50.0% to streptomycin, while the highest resistance percentage was obtained with lincocin (100.0%), followed by ampiclox (80.0%). P. aeruginosa showed the highest susceptibility percentage of 83.3% to ciprofloxacin, followed by 66.6% to streptomycin and gentamycin (66.6%), while the highest resistance percentage was obtained with streptomycin (33.3%) and gentamycin (33.3%). E. coli showed the highest susceptibility percentage to gentamycin and streptomycin with 100% activity. The antibiotics with reasonable resistant profile was observed in 10 isolates (5 S. aureus, 5 P. aeruginosa and 1 E. coli) with isolate code S1, S2, S3, S4, S5, Ps1, Ps2, Ps3, Ps4, Ps5 and Ec1 which showed resistance to atleast 5 antibiotics, hence this isolates were subjected to plasmid profile analysis. Only three isolates (S1, S4 and Ec1) showed the presence of plasmids within the range of 1.8 kbp to 10.4 kbp. Hence antibiotic resistance of an organism does not always confer the presence of plasmid.
VL - 1
IS - 6
ER -

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Author Information

Nworie O.

Department of Biological Science, Faculty of Science and Technology, Federal University Ndufu-Alike Ikwo, Ebonyi State
Nnachi A. U.

Department of Medical Microbiology and Parasitology, Faculty of Medicine, Nnamdi Azikiwe University, Awka, Anambra State
Ukaegbu C. O

Department of Microbiology, Faculty of Natural Sciences, University of Jos, Plateau State
Alo M. N.

Department of Medical Microbiology and Parasitology, Faculty of Medicine, Nnamdi Azikiwe University, Awka, Anambra State
Ekuma U. O.

Department of Applied Microbiology, Faculty of Biological Science, Ebonyi State University, Abakaliki, Ebonyi State
Ogueji E. O.

Department of Biological Science, Faculty of Science and Technology, Federal University Ndufu-Alike Ikwo, Ebonyi State

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Nworie O., Nnachi A. U., Ukaegbu C. O, Alo M. N., Ekuma U. O., et al. (2013). Antibiotic Susceptibility and Plasmid Profile of Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli Isolated from Wound Patients in Abakaliki Metropolis, Ebonyi State. American Journal of Bioscience and Bioengineering, 1(6), 75-82. https://doi.org/10.11648/j.bio.20130106.11

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Nworie O.; Nnachi A. U.; Ukaegbu C. O; Alo M. N.; Ekuma U. O., et al. Antibiotic Susceptibility and Plasmid Profile of Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli Isolated from Wound Patients in Abakaliki Metropolis, Ebonyi State. Am. J. BioSci. Bioeng. 2013, 1(6), 75-82. doi: 10.11648/j.bio.20130106.11

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Nworie O., Nnachi A. U., Ukaegbu C. O, Alo M. N., Ekuma U. O., et al. Antibiotic Susceptibility and Plasmid Profile of Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli Isolated from Wound Patients in Abakaliki Metropolis, Ebonyi State. Am J BioSci Bioeng. 2013;1(6):75-82. doi: 10.11648/j.bio.20130106.11

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@article{10.11648/j.bio.20130106.11,
  author = {Nworie O. and Nnachi A. U. and Ukaegbu C. O and Alo M. N. and Ekuma U. O. and Ogueji E. O.},
  title = {Antibiotic Susceptibility and Plasmid Profile of Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli Isolated from Wound Patients in Abakaliki Metropolis, Ebonyi State},
  journal = {American Journal of Bioscience and Bioengineering},
  volume = {1},
  number = {6},
  pages = {75-82},
  doi = {10.11648/j.bio.20130106.11},
  url = {https://doi.org/10.11648/j.bio.20130106.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20130106.11},
  abstract = {Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli has become increasingly recognized as an emerging opportunistic pathogen of clinical importance. A total of 18 isolates comprising of 10 (55.6%) S. aureus, 6 (33.3%) P. aeruginosa and 2 (11.1%) E. coli were recovered from 15 pus samples of wound patients attending Federal Teaching Hospital Abakaliki I (FETHA I) and Federal Teaching Hospital Abakaliki II (FETHA II). All the isolates were subjected to antibiotic susceptibility testing using Kirby-Bauer disk diffusion method on Mueller-Hinton agar. S. aureus showed the highest susceptibility percentage of 80.0% to ciprofloxacin, followed by 60.0% to augmentin and 50.0% to streptomycin, while the highest resistance percentage was obtained with lincocin (100.0%), followed by ampiclox (80.0%). P. aeruginosa showed the highest susceptibility percentage of 83.3% to ciprofloxacin, followed by 66.6% to streptomycin and gentamycin (66.6%), while the highest resistance percentage was obtained with streptomycin (33.3%) and gentamycin (33.3%). E. coli showed the highest susceptibility percentage to gentamycin and streptomycin with 100% activity. The antibiotics with reasonable resistant profile was observed in 10 isolates (5 S. aureus, 5 P. aeruginosa and 1 E. coli) with isolate code S1, S2, S3, S4, S5, Ps1, Ps2, Ps3, Ps4, Ps5 and Ec1 which showed resistance to atleast 5 antibiotics, hence this isolates were subjected to plasmid profile analysis. Only three isolates (S1, S4 and Ec1) showed the presence of plasmids within the range of 1.8 kbp to 10.4 kbp. Hence antibiotic resistance of an organism does not always confer the presence of plasmid.},
 year = {2013}
}

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